Yes, our 3D cell culture plates are sterilized. However, you can also add another step of sterilization using ethanol. To do so, please refer to our video guide by clicking this link.

It really depends on case by case basis. Our 3D cell culture scaffold is made from poly(ε-caprolactone), which means it has slightly negative surface charge. If this suits your application, then you can do cell seeding directly. Otherwise, you can do surface coating as if you are preparing cell seeding for 2D cell culture. Feel free to contact us if you want to discuss your specific protocol for cell attachment.

Certainly. You can remove the cells using typical trypsinization protocol. However, keep in mind that for a 3D cell culture, you may want to prolong the incubation time for the trypsinization step compared to when you are using a 2D cell culture.

You can apply general protocol for 2D cell culture in our 3D cell culture too. To stain your live cells, BCECF-AM can be used while Propidium iodide might be a good choice to stain dead cells.

You can use SEM or Confocal Microscopy to check the morphology of your samples. Also, yes, in most cases you need to take out the scaffold out of the plates for both techniques.

InoMATRIX products are compatible with both assays. However, we strongly recomend MTS assay. MTT results in formation of formazane crystals which need additional solubilization stem and fibers may be damaged during this step. For more deails please check our Protocols.

The standard thickness of InoMATRIX membrane is 200 µm. However, we offer custom fabrication of scaffolds with other thickness.

In case of nanofibrous scaffolds (NF) the pore size is about 1.5 µm. In case of microfibrous scaffolds (MF) it is about 6 µm. In case of nano/microfibrous scaffolds it is about 3 µm. In case of porous scaffolds (PF) the interfibrillar pores have size about 3 µm.However, the fibers are flexible and have high porosity (>80%) and enable cell penetration across the scaffold.

InoMATRIX products (especially Inserts) are very compatible with hydrogels. The hydrogel can be formed on surface of fibers and support the cells. Our Insert membranes are having biomimicking structure and high porosity (80% for InoMATRIX compared to 10% in case of track-etched membranes). You can use them as replacement of standard inserts with non-biomimetic surface.

InoMATRIX provides optimized 3D environment for your cells. During classical 2D cultures the cells are growing in monolayers and their morphology, expression of genes and behaviour is altered. This may alter reliability of 2D cell culture results.

The key advantages of InoMATRIX are:

• Fibrous morphology mimicking ECM – InoMATRIX Morphlology kit allows you to select optimal morphology of fibers for your cells.
• High specific surface enables efficient cell adhesion.
• Porous structure allows diffusion of nutrients – InoMATRIX has porosity >80% which keeps your cells in contact with environment.
• Flexble structure with inteconnected pores – InoMATRIX has total pore interconnection enabling cells to penetrate into the fibrous mesh.
• Easy manipulation – scaffolds have sufficient mechanical properties for manipulation during experiments.
• Large number of formats – InoMATRIX comes in form of standard microplates, inserts or custum shapes.
• Compatibility with standard methods.

Polycaprolactone has negative zeta potential and enables adhesion of ECM proteins including collagen, laminin and fibronectin.

We are offering custom scaffolds in shape based on your expectations. InoMATRIX is formed by needleless electrospinning and could be obtained in large formats (up to 500 mm wide scaffolds).

Yes. InoCure has 3D printing and CNC milling capacities for construction of organ-on-chip systems with InoMATRIX.